In this article, we compared the suitability of dry-fixation with saline versus chemical fixatives.
However, many chemicals that are widely used for cell fixation directly affect cellular biomolecules, yielding Raman spectra with missing or altered information. While live cell analysis is the most accurate approach for cellular Raman spectroscopy, the analysis of fixed cells has proved to be very useful, particularly in collaborative projects where samples need to be serially examined by different laboratories or stored and reanalyzed at a later date. Raman spectroscopy enables the label-free assessment of cellular composition.
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